![]() Furthermore, hydrocarbons biodegradation capabilities were evaluated for identified isolates through estimated degradation rate % during incubation time.Ģ.3.3. This investigation was carried out to identify isolated microbial strains from oil-contaminated soils through extensive study consisting of morphological, biochemical and molecular fingerprinting method. Firstly, designed to amplify a wide spectrum of bacterial sequences ( Teske et al., 1996, Marchesi et al., 1998). 16S rRNA specific molecular marker has been constructed to identify specific bacterial genes ( Wang et al., 1996, Wheeler et al., 1996) which dedicated to two methods. Powerful points for Molecular especially, rDNA-dependent methods to identify microorganisms are rapidity, precisely and reliability analysis of microbial cultures comparing with traditional, biochemical culture-dependent techniques, thus molecular technique has great potential for bacterial identification in new era. Recently, many advanced molecular culture-dependent techniques (like library clone, TGGE/DGGE, LH-PCR, RISA, RT-Q-PCR, FISH, RAPD and RFLP) were developed and considered a helpful tool for isolation and identification new bacterial strains with degradation capabilities ( Stancu, 2018). ( Gayathiri et al., 2017).ĭetectable conditions for Hydrocarbon degradation rates in soil, fresh water and marine environments. Eight hydrocarbon degrading bacteria were specifically detected as Alcaligen sp, Bacillus sp, Chromobacterium sp, Corynebacterium sp, Pseudomonas sp, Aeromonas sp, Serratia sp, and Flavobacterium sp. Many different factors of contaminated soil characterize influence petroleum hydrocarbons bacterial biodegradation such as pH, electrical conductivity, total nitrogen and heavy metal which are important indicators of soil quality, fertility and productivity. Petroleum hydrocarbons biodegradable illumination depends on the indigenous microorganisms to transform or mineralize the organic contaminants ( Fig. Thus, continuous evaluation for biodegradation rate considered a critical needed for different biodegradable microorganisms ( Alquati et al., 2005). One of the initiative efficient, economical and environmental treatment mechanisms for petroleum biodegradation is In situ biodegradation through degrade petroleum and other hydrocarbons from culture via widely distributed microorganisms and applied for varied hydrocarbon-contaminated soils and waters ( Margesin and Schinner, 1997, Whyte et al., 1997). However, bacteria considered important biodegrable microorganisms which play a critical role in hydrocarbon degradation ( Udgire et al., 2015). Different microorganisms could be applied for Petroleum hydrocarbons biodegradation. 50–98% of crude oil is Petroleum hydrocarbons which considered a major component depending on the source of the oil. Petroleum (crude) oil are compounded of thousands compounds mixture. Remarkable degradation rate % patterns for hydrocarbons residual were recorded for all three isolates with varied. Different features were studied for three biodegrading bacterial strains and identified as Bacillus subtilis, Pseudomonas aeruginosa and Bacillus cereus. Biodegradation patterns for different three isolates were reflected varied degradation ability for three isolates regarding incubation time. Phyllogenetic tree was constructed and genetic similarity was calculated according to alignments results. Interestingly, 16S rDNA sequencing fingerprinting results confirmed our bacterial identification as Bacillus subtilis, Pseudomonas aeruginosa and Bacillus cereu. Three bacterial strains were selected from petroleum-contaminated soils of Khurais oil field depending on their capacity to grow in the existence of hydrocarbon components and identified according to morphological, biochemical. Furthermore, degradation level in contaminated liquid medium and soil were evaluated. The aim of this study is isolation and identification biodegradable capability bacteria from contaminated sites in Khurais oil field, Dhahran, Saud Arabia via Different morphological and biochemical and molecular methods. Until now, In situ biodegradation considered the most effective method for petroleum hydrocarbon residual biodegradation. Accumulation of petroleum hydrocarbon residual considered a major environmental problem in the kingdom of Saudi Arabia cause of intensive efforts for oil detecting.
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